Figure 7.

Immunization with IDLV-tbsHTI Does Not Affect the HTI-Specific Immune Response

BALB/c mice were immunized with SIV-based sIDLV-tbsHTI (black bar), sIDLV-HTI (gray bar), or sIDLV-GFP (hatched bar). The specific T cell response was evaluated by IFNγ ELISpot at 30 days after immunization. Cells were stimulated overnight with the H-2d-restricted epitopes Gag-9-mer and GFP-9-mer or 10 pools of peptides spanning the whole HTI sequence (P1–P10) (Table S1). Data are expressed as specific spot-forming cells (SFCs) per 10⁶ splenocytes. Error bars represent the standard deviation among animals from the same group.