Value of the Data •Nusbiarylins are the first-in-class bacterial ribosomal RNA synthesis inhibitors that possess potent activity against various types of multidrug-resistant bacteria with a novel mode of action •Spectral data of nusbiarylins are useful for elucidating their purity •The HPLC profiles, qNMR and HRMS spectroscopic data are of 95 unreported compounds and could be useful for the characterization by other researchers