Table 1.
RNA Sequences of DENV2 Targeted by CRISPR-Cas13a
| Target | RNA Sequence (5′→3′) | Sitea |
|---|---|---|
| crRNA-capsid | UCAAUAUGCUGAAACGCGAGAGAAACCG | 133–161 |
| crRNA-PrM | AACAUGGAUGUCAUCAGAAGGGGCCUGG | 761–789 |
| crRNA-NS1 | CGUGCACACAUGGACAGAACAAUACAA | 2491–2519 |
| crRNA-NS2a | UUACAAAACGCAUGGAAAGUGAGUUGCA | 3955–3985 |
| crRNA-NS2b | AUGAUAUUCCCAUGACAGGACCAUUAGU | 4202–4230 |
| crRNA-NS3 | UCCAUACAAUGUGGCAUGUCACACGUGG | 4657–4685 |
| crRNA-NS4a | AAUUUGCAGCCGGAAGAAAGUCUCUGAC | 6355–6383 |
| crRNA-NS4b | CAAAAGCAACCAGAGAAGCUCAGAAAAG | 7204–7233 |
| crRNA-NS5 | GUGUGUACAACAUGAUGGGAAAAAGAGA | 8917–8945 |
| crRNA-3′UTR | AACAAAAAACAGCAUAUUGACGCUGGGA | 10647–10675 |
a
Site of sequence was obtained from GenBank: U87411 (DENV-2).