Table 1.
Thermal inactivation kinetics and thermodynamic parameters of free and Ch-MNP /PEI/GA/Enzym.
| Enzyme | Ed (KJ/mol) | Thermal inactivation parameters |
Temperature (°C) |
Thermodynamic parameters |
Temperature (°C) |
||
|---|---|---|---|---|---|---|---|
| 60 | 70 | 60 | 70 | ||||
| Free | 23.42 | Kd (min−1) | 10.25 × 10−3 | 11.55 × 10−3 | ΔH° (kJ/mol) | 20.65 | 20.57 |
| t1/2 (min) | 67.60 | 59.99 | ΔG° (kJ/mol) | 92.56 | 95.09 | ||
| D-value (min) | 224.56 | 199.30 | ΔS° (J/mol/°K) | −0.22 | −0.22 | ||
| Immobilized | 88.98 | Kd (min−1) | 0.47 × 10−3 | 0.87 × 10−3 | ΔH° (kJ/mol) | 86.21 | 86.13 |
| t1/2 (min) | 1472.98 | 795.34 | ΔG° (kJ/mol) | 100.91 | 102.31 | ||
| D-value (min) | 4893.15 | 2642.05 | ΔS° (J/mol/°K) | −0.05 | −0.05 | ||