Figure 4.

GAS5-Induced ADR Sensitivity of Breast Cancer Cells Was Abated by miR-221-3p Upregulation

MCF-7/ADR cells were transfected with pcDNA-GAS5 alone or together with miR-221-3p mimics, and MCF-7 cells were transfected with si-GAS5#1 alone or together with anti-miR-221-3p. (A) The miR-221-3p expression in MCF-7/ADR and MCF-7 cells was examined by quantitative real-time PCR. (B) IC₅₀ of ADR and cell proliferation capacity were measured by a CCK-8 assay in MCF-7/ADR and MCF-7 cells. (C) The colony-forming ability was measured by a colony-forming assay. (D) Cell apoptosis was evaluated by flow cytometry. The data are shown as the mean ± SD (n = 3). *p < 0.05, **p < 0.01 versus respective control.