Figure 5.

miR-221-3p Targets the 3′ UTR of DKK2 and Regulates the ABCB1 Expression

(A) Putative binding sites between DKK2 and miR-221-3p predicted by the TargetScan online website together with the mutant sites of the DKK2-3′ UTR region in the MUT-DKK2-3′ UTR reporter vector. (B) The luciferase activity was detected in MCF-7/ADR cells transfected with DKK2-WT or DKK2-MUT and miR-221-3p mimics or anti-miR-221-3p. (C) The DKK2 mRNA expression in breast cancer tissues was examined by quantitative real-time PCR. (D) The DKK2 mRNA expression in MCF-7 and MCF-7/ADR cells was examined by quantitative real-time PCR. (E) The MCF-7/ADR cells were transfected with miR-221-3p mimics or anti-miR-221-3p. The DKK2 and ABCB1 mRNA expression levels were examined by quantitative real-time PCR. (F) The DKK2 and ABCB1 protein expression levels were examined by western blot analysis. Data are shown as the mean ± SD (n = 3). *p < 0.05, **p < 0.01 versus respective control.