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. 2020 Feb 1;19:1434–1448. doi: 10.1016/j.omtn.2020.01.030

Figure 7.

Figure 7

Overexpression of GAS5-Sensitized MCF-7/ADR Cells to ADR In Vivo

MCF-7/ADR cells stably infected pcDNA-GAS5 or pcDNA-NC were subcutaneously injected into nude mice, followed by administration with ADR (4 mg/kg) every 4 days from the 7th day after cell inoculation. (A) Tumor volumes were examined every 4 days for 27 days. (B) Representative photographs and average weights of dissected tumors. (C) The GAS5 expression in resected tumor masses was detected by quantitative real-time PCR. (D) The miR-221-3p expression in resected tumor masses was detected by quantitative real-time PCR. (E) The mRNA expression levels of DKK2 and ABCB1 in resected tumor masses were detected by quantitative real-time PCR. (F) The protein expression levels of DKK2 and ABCB1 in resected tumor masses were detected by western blot analysis. Data are shown as the mean ± SD (n = 3). *p < 0.05, **p < 0.01 versus respective control.