Table 3.

Operetta High-Content Imaging System settings and values of minimum intensity of positive cells.

Setting	Conditions
Plate type	PerkinElmer Cell Carrier 96
Objective	20× long WD
Opt. mode	Non-confocal
Excitation	100%
Calculate program	Intensity cell Alexa 488, 568 mean – inner cells

Target	PECAM1	CDH5	CD34	Ki-67	Ac-LDL	β-Gal
Figure 2C (610B1)	750	350	200
Figure 2C (608A1)	850	450	160
Figure 2C (648A1)	720	300	135
Figure 2D (610B1)					930
Figure 2D (606A1)					450
Figure 2D (648A1)					450
Figure 3C	1270	550	550
Figure 3D					700
Figure 8B						3000
Figure 9E				500
Figure 9F					500