Figure 4.
Effects of each herbal crude drug containing NYT on the increase in impedance of OX1R-expressing HEK293 cells. (A) HEK293 cells stably expressing OX1R were treated with each herbal crude drug alone contained in NYT (20 μg/ml) or its vehicle (control) (n = 6–12). (B) The cells expressing OX1R were pretreated with or without the selective OX1R antagonist SB676042 (SB, 10−6 or 10−5 M) for 30 min and then treated with citrus unshiu peel (20 μg/ml) (n = 5–6). The rate of change in impedance is expressed as the difference of the minimum and maximum impedance after drug injection. The data are expressed as mean ± S.E.M (n = 6). ** < 0.001, and ****p < 0.0001, respectively, compared with the vehicle (control); #p < 0.05, compared with citrus unshiu peel; Bonferroni's multiple comparison test following one-way ANOVA.