TABLE 1.
Strains and plasmids used in this study.
| Strain | Characteristics |
| E. coli DH5α1 | supE44ΔlacU169(Φ80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 |
| P. taiwanensis VLB1202 | Solvent tolerant, styrene degrading bacterium, isolated from forest soil |
| Plasmids | |
| pCom10_lac_Cyp | pRO1600 and ColE1ori, lac-regulatory system (lacI, PlacUV5), KmR, pCom10 RBS, Cyp genes from Acidovorax sp. |
| pCom10_tac_Cyp | pRO1600 and ColE1ori, lac-regulatory system (lacIq, Ptac), KmR, pCom10 RBS, Cyp genes from Acidovorax sp. |
| pSEVA244_BB32_Cyp | pRO1600 and ColE1ori, lac-regulatory system (lacIq, Ptrc), BBa_B0032 RBS, Cyp genes from Acidovorax sp. |
| pSEVA244_BB34_Cyp | pRO1600 and ColE1ori, lac-regulatory system (lacIq, Ptrc), BBa_B0034 RBS, Cyp genes from Acidovorax sp. |
| pSEVA244_RBS* _Cyp | pRO1600 and ColE1ori, lac-regulatory system (lacIq, Ptrc), RBS*, Cyp genes from Acidovorax sp. |
| pSEVA254_BB32_Cyp | RSF1010 ori, lac-regulatory system (lacIq, Ptrc), BBa_B0032 RBS, Cyp genes from Acidovorax sp. |
| pSEVA254_BB34_Cyp | RSF1010 ori, lac-regulatory system (lacIq, Ptrc), BBa_B0034 RBS, Cyp genes from Acidovorax sp. |
| pSEVA254_RBS* _Cyp | RSF1010 ori, lac-regulatory system (lacIq, Ptrc), RBS*, Cyp genes from Acidovorax sp. |
| pSEVA244_T_BB34_Cyp | pRO1600 and ColE1ori, lac-regulatory system (lacIq, Ptrc), BBa_B0015 terminator, BBa_B0034 RBS, Cyp genes from Acidovorax sp. |
| pSEVA244_T_RBS* _Cyp = pSEVA_Cyp | RSF1010 ori, lac-regulatory system (lacIq, Ptac), RBS*, Cyp genes from Acidovorax sp. |
| pCom10_Cyp3 | pRO1600 and ColE1 ori, alk-regulatory system (alkS, PalkB), KmR, pCom10 RBS, Cyp genes from Acidovorax sp. |
1Hanahan, 1983. 2Köhler et al., 2013. 3Karande et al., 2016. If not stated otherwise, the plasmids and strains were constructed in this study.