TABLE 2.
Specific whole-cell activities, Cyp concentrations, and Cyp turnover numbers obtained with the different expression vectors constructed.
| Plasmid backbone | RBS | Activity (U gCDW–1) | Active Cyp concentration1 (nmol gCDW–1) | Turnover number (mol min–1 mol–1) |
| ColE1/pRO1600_alk | Default pCom | 22.2 ± 1.2 | 50.6 ± 1.9 | 439.4 ± 40.4 |
| ColE1/pRO1600_lac | Default pCom | 35.0 ± 1.9 | 76.7 ± 11.7 | 455.6 ± 95.0 |
| ColE1/pRO1600_tac | Default pCom | 18.5 ± 1.0 | 42.8 ± 5.7 | 432.5 ± 81.1 |
| ColE1/pRO1600 | Weak | 14.0 ± 0.9 | 25.4 ± 0.3 | 551.3 ± 29.0 |
| Moderate | 48.4 ± 1.5 | 78.9 ± 4.2 | 613.7 ± 51.7 | |
| Strong | 4.7 ± 0.3 | 46.1 ± 9.6 | 101.5 ± 28.0 | |
| RSF1010 | Weak | 2.7 ± 0.7 | 11.3 ± 9.8 | 242.1 ± 266.3 |
| Moderate | 50.7 ± 0.7 | 67.7 ± 7.6 | 749.5 ± 94.1 | |
| Strong | 49.8 ± 4.6 | 76.7 ± 11.7 | 665.5 ± 85.3 | |
| ColE1/pRO1600 with terminator | Moderate | 42.4 ± 2.4 | 78.1 ± 11.7 | 543.3 ± 113.0 |
| Strong | 27.3 ± 2.4 | 81.1 ± 6.2 | 336.7 ± 55.1 |
1CYP concentrations were obtained by CO difference spectroscopy.