TABLE 4.
Literature data for cyclohexane hydroxylations.
| Enzyme | Catalyst format | additives | Temperature (°C) | Reaction time | Product formation rate (mol min–1 mol–1) | Total turnover number (-) | Initial activity (U gCDW–1) | Specific yield (mmol gCDW–1) | References |
| sMMOH | Purified enzyme | H2O2 | 45 | 10 min | 0.00014 | – | – | – | Jiang et al., 1993 |
| P450 BM3 | Purified enzyme | – | RT | initial | 151 | – | – | – | Glieder et al., 2002 |
| P450 BM3 | Purified enzyme | PFC9a | 25 | 10 min | 108 | – | – | – | Kawakami et al., 2011 |
| Modified P450 BM3 | Purified enzyme | – | RT | initial | 3910b | – | – | – | Glieder et al., 2002 |
| Modified CYP153A13a | Purified enzyme | – | RT | 2 h | 3 | 20 | – | – | Bordeaux et al., 2011 |
| P.taiwanensis VLB120 pCom10_Cyp | Whole cells | – | 30 | 3 h | 336c | 22 711 | 20c | 1.42d | This study |
| P.taiwanensis VLB120 pSEVA_Cyp | Whole cells | – | 30 | 3 h | 543c | 18 628 | 55c | 2.46d | This study |
| Syn6803_CYP | Whole cells | DINPe | 30 | 52 h | – | – | 35c | 49 | Hoschek et al., 2019 |
aPerfluorocarboxylic acid with nine carbon atoms acts as dummy substrate to provide space for cyclohexane because of the incomplete occupation of the heme cavity. bCalculated from photospectrometrically recorded NADPH depletion including possible uncoupling. cData for the first 10 min of the reaction. dAddition of 5 mM cyclohexane as substrate. eDiisononyl phthalate added as an organic phase.