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. 2020 Feb 27;11:97. doi: 10.3389/fgene.2020.00097

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Copyright © 2020 Pletnev, Guseva, Zanina, Evfratov, Dzama, Treshin, Pogorel’skaya, Osterman, Golovina, Rubtsova, Serebryakova, Pobeguts, Govorun, Bogdanov, Dontsova and Sergiev

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Influence of rRNA methyltransferase gene inactivation on the expression of exogenous reporter genes encoded on the plasmid carrying red fluorescent protein (RFP) gene under the control of a constitutive T5 promoter and cerulean fluorescent protein (CER) gene under a control of an induced Tet promoter. Shown are the intensities of the CER (blue) and RFP (orange) fluorescence in the overnight cultures of the strains devoid of the 16S rRNA (A) and 23S rRNA (B) methyltransferase genes as labeled below the graphs. WT corresponds to the parental isogeneic strain carrying all set of rRNA methyltransferase genes. IND marks the graphs corresponding to the induction of CER gene expression by anhydrotetracycline.