Table 1.

List of E. coli rRNA MT coding genes and the phenotypes of their knockouts.

Nucleotide	Enzyme	Reference	Growth of knockout strain/growth at overexpression*	CER expression**	FastFT expression***	Accumulation of assembly intermediates and 17S rRNA precursor	Expression level**** and timing
16S rRNA
527 m7G	RsmG	Okamoto et al., 2007	+++\++	+++	+++	−/−	+++, 3h
966 m2G	RsmD	Lesnyak et al., 2007	+++\+++	+	+++	−/−	+++, 2h
967 m5C	RsmB	Tscherne et al., 1999a; Gu et al., 1999	+++\+++	++	++	−/++	++++, 4h
1207 m2G	RsmC	Tscherne et al., 1999b	+++\+++	++	+++	−/−	++++, 2h
1402 m4Cm	RsmI, RsmH	Kimura and Suzuki, 2010	+++\+	+	+/+++#	−/−	+, 2h
1407 m5C	RsmF	Andersen and Douthwaite, 2006	+++\+++	++++	++++	−/−	+++, 2h
1498 m3U	RsmE	Basturea et al., 2006	+++\+++	+++	+++	−/−	+++, 2h
1516 m2G	RsmJ	Basturea et al., 2012	+++\+++	+	++	−/−	+++, 2h
1518/9 m6 2A	RsmA	Helser et al., 1972; Poldermans et al., 1979	+++\+++	+	+	−/++	++++, 2h
23S rRNA
745 m1G	RlmA	Gustafsson and Persson, 1998	+++\++	+	+/+++#	−/−.	++++, 2h
747 m5U	RlmC	Madsen et al., 2003	+++\++	+	+++	−/++	++, 2h
1618 m6A	RlmF	Sergiev et al., 2008	+++\++	+	+++	−/−	+++, 1–7h
1835 m2G	RlmG	Sergiev et al., 2006	+++\+++	++	++	−/−	+++, 2h
1915 m3 Ψ	RlmH	Purta et al., 2008a; Ero et al., 2008	+++\+++	+	++	−/−	+++, 2h
1939 m5U	RlmD	Agarwalla et al., 2002; Madsen et al., 2003	+++\+++	+++	+++	−/−	+++, 2h
1962 m5C	RlmI	Purta et al., 2008b	+++\+	+	++	−/−	+++, 2h
2030 m6A	RlmJ	Golovina et al., 2012	+++\+++	+	+++	−/−	++++, 2h
2069 m7G, 2445 m2G	RlmKL	Kimura et al., 2012	+++\+++	+	+++	−/−	+++, 2h
2251 Gm	RlmB	Lovgren and Wikstrom, 2001	+++\+++	++	+++	−/−	+++, 2h
2498 Cm	RlmM	Purta et al., 2009	+++\+++	+	++	+?/−	+++, 2h
2503 m2A	RlmN	Toh and Mankin, 2008	+++\++	+	+++	+?/-	+++, 2h
2552 Um	RlmE	Caldas et al., 2000; Bugl et al., 2000	+\+	+	+/++#	+++/−	++++, 3h
	WT		+++	+++	+++	−/−

*+++ corresponds to doubling times 40–60 min, ++ 60–70 min, + > 70 min. ** CER fluorescence after overnight growth at inducing conditions ++++ > 6·10⁵, +++ 4−6·10⁵, ++ 2−4·10⁵, + < 4·10⁵. ***Fluorescence intensity of the blue, newly produced form of the FastFT at the exponential growth conditions ++++ > 2·10³, +++1–2·10³, ++5·10²–10³, + < 5·10². ****Expression levels at a timepoint with maximal expression. ++++ > 10⁻⁴, +++ > 10⁻⁵, ++ > 10⁻⁶, + < 10⁻⁶ relative to the 16S rRNA. ^#During growth of these strains transformed by FastFT expression plasmid a significant amount of non-fluorescent cells were accumulated.