FIGURE 5.

(A) Expression of COMP, ITGB8, TP53, EGFR, and EGFR^L858R in H1299 and A549 cells after transfection. (B) CCK-8 assay detected the proliferation by the value of OD₄₉₀nm. (C) Representative cell colony formation assay and quantitative representation detected the proliferation of H1299 and A549 cells after transfection with EGFR^L858R or NC in A549 or H1299. (D) Representative images of initial and final wounds in A549 or H1299 cells after transfection with EGFR^L858R and negative control by wound-healing assay (Scale bar: 200 μm). (E) Representative transwell migration assays and quantitative representation after transfection with EGFRL858R or NC in A549 or H1299 cells (Scale bar: 100 μm). Assays were performed in triplicate. ^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗P < 0.001. Error bars represent SEM. NC, negative control; WT, wild-type; MUT, mutant type.