Table 3.

In vitro antibacterial, antifungal and cytotoxic activity of dematophoranes A-B (1-2), libertellenone M (3), myrocin B (5) and libertellenone C (7). All compounds were dissolved in methanol (1 mg/ml, test volume: 20 μl). 20 μl of methanol showed no effect on the test organisms. MIC: Minimum inhibitory concentration. Positive controls: [a] Oxytetracyclin hydrochloride, [b] Gentamycin, [c] Nystatin; [d] Epothilon B, n.i.: no inhibition.

Test organisms	MIC [μg/mL]
	(1)	(2)	(3)	(5)	(7)	Ref [a,b,c]
Gram-positive bacteria
Bacillus subtilis DSM 10	n.i	67.0	n.i	33.3	n.i.	8.3 [a]
Micrococcus luteus DSM 1790	67.0∗	67.0	n.i	67.0	n.i	0.42 - 0.83 [a]
Mycobacterium smegmatis ATCC700084	67.0	67.0	n.i	67.0	n.i.	3.3 [a]
Staphylococcus aureus DSM 346	67.0	67.0	n.i	33.3	67.0∗	0.42–0.83 [a]
Gram-negative bacteria
Chromobacterium violaceum DSM 30191	n.i	n.i	n.i	n.i	n.i	0.83 [a]
Escherichia coli DSM 1116	n.i	n.i	n.i	n.i	n.i	3.3–6.7 [a]
Pseudomonas aeruginosa PA 14	n.i	n.i	n.i	n.i	n.i	4.2 [b]
Yeasts
Candida albicans DSM 1665	n.i	n.i	n.i	n.i	n.i	8.3 [c]
Wickerhamomyces anomalus DSM 6766	n.i	n.i	n.i	-	n.i	16.6 [c]
Rhodotorula glutinis DSM 10134	n.i.	n.i.	n.i.	n.i	n.i	2.1 [c]
Schizosaccharomyces pombe DSM 70572	n.i	n.i	n.i	n.i	n.i	16.6 [c]
Filamentous fungi
Mucor hiemalis DSM 2656	n.i.	67.0∗	67.0∗	n.i	67.0∗	8.3 [c]
Cell lines
Mouse fibroblast cell line L929	28	20	6.3	6.5	9.5	1.1 × 10-3 [d]
Cervix carcinoma cell line KB-3-1	6.5	16	6.1	-	14	0.06 × 10-3 [d]
Primary Human Umbilical Vein Endothelial Cells HUVEC	-	-	-	0.75	-	0.2 × 10-3 [d]

The cell density was adjusted to 8 × 10⁶ cells/mL. ∗ no complete inhibition; DSMZ: German Collection of Microorganisms and Cell Cultures, Braunschweig.