Figure 1.

Workflow of M-IMC. (A) After tissue staining with metal tagged antibodies on TMA, first step was to create images. After single-cell segmentation of tissue images, higher-order analysis was performed to visualize results such as t-SNE maps. (B) The element of each metal tagged antibody is listed. Five EMT markers: α-SMA, vimentin, cytokeratin 7, pan-keratin, and collagen I. One tumor proliferation marker: Ki-67. One immune cell marker: HLA-DR. Two nuclear counterstainings. (C, D, and E) The expression of each target was generated by heat map; heat map of tumor grade (C), TNM stage (D), tumor stage (E).