Fig. 3.

Metabolic fate of the ingested aliphatic and indolic GLS in P. armoraciae adults. The amount of aliphatic and/or indolic GLS ingested from Arabidopsis wild type, myb, and cyp rosette leaves, respectively, was set to 100%, and the corresponding percentages of aliphatic and indolic GLS detected in beetles and feces were calculated. The plots show the accumulated and excreted proportions of ingested aliphatic and/or indolic GLS by P. armoraciae adults relative to the total ingested GLS amount per beetle. a Accumulation of aliphatic and indolic GLS ingested from Arabidopsis wild type. b Accumulation of aliphatic and indolic GLS ingested from the cyp or myb mutant, respectively. c Accumulation of aliphatic GLS ingested from wild type plant and cyp mutant, respectively. d Accumulation of indolic GLS ingested from wild type plant and myb mutant, respectively. e Excretion of aliphatic and indolic GLS ingested from Arabidopsis wild type. f Excretion of aliphatic and indolic GLS ingested from cyp and myb mutant, respectively. g Excretion of aliphatic GLS ingested from wild type plant and cyp mutant, respectively. h Excretion of indolic GLS ingested from wild type plant and myb mutant, respectively. The statistical analyses were performed by ANCOVA, generalized least squares method, or linear mixed effects models using the total ingested GLS amount per beetle as covariable, with linear or quadratic regression. If necessary, data were transformed prior to analysis. The final estimates were back-transformed to their original scale, and plotted with regression lines. Data series that are not significantly different from each other were combined for regression line plotting. For methods and results of the statistical analyses, refer to Table 2. Aliphatic GLS: sum of 3MSOP GLS, 4MSOB GLS, 4MTB GLS, 5MSOP GLS, 7MSOH GLS, 8MSOO GLS; indolic GLS: sum of I3M GLS, 1MOI3M GLS, 4MOI3M GLS. For plant and GLS abbreviations, refer to legend of Figs. 1 and 2, respectively