Table 2.
Methods and results of statistical analyses of metabolic fate of ingested aliphatic and indolic GLS in P. armoraciae adults
| Metabolic fate | Comparison | Statistical method | Equation form | Variance structure | Variable | Transformation | Statistics | P value |
|---|---|---|---|---|---|---|---|---|
| Accumulation | Aliphatic and indolic GLS from Arabidopsis wild type (Fig. 3a) | Linear mixed effects model | Linear | – | Accumulated percentage | – | – | – |
| Ingested GLS amount | – | LR = 8.790 | = 0.003 | |||||
| GLS type | – | LR = 27.778 | < 0.001 | |||||
| Interaction | – | LR = 6.860 | = 0.009 | |||||
| Aliphatic GLS from cyp mutant, indolic GLS from myb mutant (Fig. 3b) | ANCOVA | Linear | – | Accumulated percentage | Arcsin-square-root | – | – | |
| Ingested GLS amount | Log | F = 7.631 | = 0.013 | |||||
| Plant | – | F = 9.160 | = 0.008 | |||||
| Interaction | – | F = 0.687 | = 0.420 | |||||
| Aliphatic GLS from Arabidopsis wild type and cyp mutant (Fig. 3c) | Generalized least squares | Linear | varComb (varIdent (form = ~ 1 | plant),varFixed(~ Ingested GLS amount)) | Accumulated percentage | Arcsin-square-root | – | – | |
| Ingested GLS amount | Log | LR = 24.058 | < 0.001 | |||||
| Plant | – | LR = 3.625 | = 0.057 | |||||
| Interaction | – | LR = 0.150 | = 0.698 | |||||
| Indolic GLS from Arabidopsis wild type and myb mutant (Fig. 3d) | Generalized least squares | Linear | varPower (form = ~ log (Ingested GLS amount)) | Accumulated percentage | Arcsin-square-root | – | – | |
| Ingested GLS amount | Log | LR = 26.602 | < 0.001 | |||||
| Plant | – | LR = 1.095 | = 0.295 | |||||
| Interaction | – | LR = 0.560 | = 0.454 | |||||
| Excretion | Aliphatic and indolic GLS from Arabidopsis wild type (Fig. 3e) | Linear mixed effects model | Linear | – | Excreted percentage | Arcsin-square-root | – | – |
| Ingested GLS amount | Log | LR = 5.279 | = 0.022 | |||||
| GLS type | – | LR = 13.807 | < 0.001 | |||||
| Interaction | – | LR = 3.313 | = 0.069 | |||||
| Aliphatic GLS from cyp mutant, indolic GLS from myb mutant (Fig. 3f) | Generalized least squares | Linear | varExp (form = ~ Ingested GLS amount | plant) | Excreted percentage | – | – | – | |
| Ingested GLS amount | – | LR = 0.002 | = 0.964 | |||||
| Plant | – | LR = 15.311 | < 0.001 | |||||
| Interaction | – | LR = 0.321 | = 0.571 | |||||
| Aliphatic GLS from Arabidopsis wild type and cyp mutant (Fig. 3g) | ANCOVA | Linear | – | Excreted percentage | – | – | – | |
| Ingested GLS amount | – | F = 3.413 | = 0.082 | |||||
| Plant | – | F = 8.194 | = 0.010 | |||||
| Interaction | – | F = 0.142 | = 0.711 | |||||
| Indolic GLS from Arabidopsis wild type and myb mutant (Fig. 3h) | Generalized least squares | Quadratic | varExp (form = ~ Ingested GLS amount | plant) | Excreted percentage | – | – | – | |
| Ingested GLS amount | – | LR = 6.809 | 0.009 | |||||
| Plant | – | LR = 8.359 | 0.004 | |||||
| Ingested GLS amount: Plant Interaction | – | LR = 6.095 | 0.014 | |||||
| Ingested GLS amount2 | – | LR = 5.659 | 0.017 | |||||
| Ingested GLS amount2:Plant Interaction | – | LR = 0.062 | 0.803 |
LR, likelihood ratio