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. 2020 Jan 9;37(2):459–469. doi: 10.1007/s10815-019-01661-0

Table 1.

Sequences of the sequence-tagged site (STSs) primers used for deletions analysis

Region Primers Forward Reverse Product size (bp) Annealing temperature (°C)
AZFa SY84 5′-AGA AGG GTC CTG AAA GCA GGT-3′ 5′-GCC TAC TAC CTG GAG GCT TC-3′ 326 57
SY86 5′-GTG ACA CAC AGA CTA TGC TTC-3′ 5′- ACA CAC AGA GGG ACA ACC CT - 3′ 318 56
AZFb SY127 5′-GGC TCA CAA ACG AAA AGA AA-3′ 5′-CTG CAG GCA GTA ATA AGG GA-3′ 274 56
SY134 5′-GTC TGC CTC ACC ATA AAA CG-3′ 5′-ACC ACT GCC AAA ACT TTC AA-3′ 301 57
AZFc SY254 5′-GGG TGT TAC CAG AAG GCA AA-3′ 5′-GAA CCG TAT CTA CCA AAG CAG C-3′ 380 57
SY255 5′-GTT ACA GGA TTC GGC GTG AT-3′ 5′-CTC GTC ATG TGC AGC CAC-3′ 123 57
Internal controls SRY 5′-GAA TAT TCC CGC TCT CCG GA-3′ 5′-GCT GGT GCT CCA TTC TTG AG-3′ 472 57
ZFX/Y 5′-ACC RCT GTA CTG ACT GTG ATT ACA C-3′ 5′-GCA CYT CTT TGG TAT CYG AGA AAG T-3′ 495 57