TABLE 2.
Mating assays
| Purpose of mating assay | Donor | Recipient | Selection, mediuma | Transconjugant |
|---|---|---|---|---|
| Transfer of pEA409TEM24 into Pm294MATLI | UB1637rif/pEA409TEM24 | Pm294MATLI (SGI1-LK1) | CHL + CAZ, MacConkey | Pm294MATLI (SGI1-LK1)/pEA409TEM24 |
| Transfer of SGI1-LK1 into E. coli UB1637rif | Pm294MATLI (SGI1-LK1)/pEA409TEM24 | UB1637rif | RIF + CHL, MacConkey | UB1637rif SGI1-LK1 |
| Transfer of pEA409TEM24 into E. coli UB1637rif SGI1-LK1 | UB5201/pEA409TEM24 | UB1637rif SGI1-LK1 | RIF + TET + CAZ, LBA | UB1637rif SGI1-LK1/pEA409TEM24 |
| Measurement of transfer rate | UB1637rif SGI1-LK1/pEA409TEM24 | UB5201 | RIF (donor) and NAL + TET (transconjugant), LBA | UB5201 SGI1-LK1 |
a
The following antibiotics were used: nalidixic acid (NAL) (25 mg/liter) for selecting UB5201, rifampin (RIF) (50 mg/liter) for selecting UB1637rif, chloramphenicol (CHL) (50 mg/liter) or tetracycline (TET) (10 mg/liter) for selecting SGI1-LK1, and ceftazidime (CAZ) (4 mg/liter) for selecting the plasmid pEA409TEM24. LBA, Luria-Bertani agar medium.