Supplementary Figure S4.

Validation of all circRNA isoforms at different gene loci detected by CircAST Schematic models of all circRNA isoforms of Csnk1d (Chr11:120,967,995–120,973,969) (A), AW554918 (Chr18:25,339,714–25,420,075) (B), Stau2 (Chr1:16,440,323–16,509,408) (C), and Dcaf8 (Chr1:172,173,943–172,187,460) (D) are provided separately (top rows on the left). The RT-PCR results (on the right) for each circRNA isoform and the corresponding Sanger sequencing data showing correct specific splicing sites (bottom rows on the left) are provided for validation. All circRNA isoforms in the four gene loci were validated successfully except circDcaf8-1-2.