Supplementary Figure S5.

Validation of the circRNA isoforms at different gene loci detected by CircAST but missed by CIRCexplorer2 Schematic models of circRNA isoforms of Cep350 (Chr1:155,953,154–155,962,560) (A), Eya3 (Chr4:132,656,693–132,673,032) (B), and Crem (Chr18:3,287,904–3,327,591) (C) are provided separately (top rows on the left). The RT-PCR results (on the right) for each circRNA isoform and the corresponding Sanger sequencing data showing correct specific splicing sites (bottom rows on the left) are provided for validation. All three isoforms were validated successfully. D.Gapdh was used as a negative control.