Validation of the circRNA isoforms in different gene loci predicted by CIRCexplorer2 but missed by CircAST The schematic models of circRNA isoforms of Drc7 (Chr8:95,061,713–95,062,395) (A), Uggt2 (Chr14:118,994,946–119,002,986) (B), Agtpbp1 (Chr13:59,473,688–59,482,604) (C), Adam3 (Chr8:24,719,415–24,725,361) (D), Lin54 (Chr5:100,475,689–100,485,855) (E), Usp32 (Chr11:85,017,630–85,022,905) (F), Mllt10 (Chr2:18,101,458–18,126,229) (G), and Scaper (Chr9:55,912,040–55,921,338) (H) are provided separately (on the left). The RT-PCR results for each circRNA isoform showing incorrect product size are provided separately (on the right). These eight circRNA isoforms failed validation.