Supplementary Figure S9.

Performance of CircAST on different lengths of sequencing reads trimmed from PE250 data (SRR7350933) used in CIRI-full A. CircAST with PE100 reconstructed 61.42% circRNAs of CIRI-full results, and its performance got lower as the sequencing reads became longer; B. CircAST missed mostly intronic or intergenic circular isoforms, and CIRI-full missed mostly longer exonic circular isoforms with length ≥ 600 bp. These two methods have different advantages in identifying various types of circular isoforms.