. 2020 Jan 8;10(3):1029–1037. doi: 10.1534/g3.119.401035

Table 3. Identification of deletion mutations in F₁ adults.

Gene	Forward primer	Reverse primer	Expected PCR size bp	Mutation type	Distance bp	# F₁ adults with an LKO deletion	# F₁ adults analyzed	% F₁ adults with an LKO deletion
*smarca2*	Fw 1	Rv 3	340-430	—	—	0	154	0
	Fw 1	Rv 15	∼310	LKO	78, 224	3	154	1.95
	Fw 1	Rv 28	250-320	—	–	0	154	0
	Fw 15	Rv 28	260-410	LKO	49,644	5	154	3.25
	Fw 3	Rv 3	∼340	—	—	0	154	0
	Fw 15	Rv 15	∼240	SKO	77	11	154	7.14
	Fw 28	Rv 28	∼280	SKO	66	4	154	0.26
*rnf185*	Fw 2	Rv 4	∼240	LKO	3,366	2	48	4.2
*rnf215*	Fw 6	Rv 7	∼320	LKO	1,037	3	48	6.25

Note: SKO: small knockout; LKO, large knockout.

Table 3. Identification of deletion mutations in F1 adults.