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. 2020 Jan 14;10(3):1039–1050. doi: 10.1534/g3.119.400813

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Copyright © 2020 Deecker et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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A comparison of the Lens chromosome and pLPL CRISPR-Cas systems. A) The overall pairwise amino acid identity across the Cas proteins of the two systems is approximately 97%, with individual pairwise Cas protein identities ranging from 96 to 99%. B) A CRISPRStudio alignment of the arrays for two systems shows completely unique spacer content and a differing number of spacers. C) Analysis of the repeat sequences shows that the Lens pLPL and chromosome systems have one SNP between their consensus repeats, in addition to possessing a mutated last repeat in their arrays. Mutations are denoted in red.