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. 2020 Jan 14;10(3):1039–1050. doi: 10.1534/g3.119.400813

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Copyright © 2020 Deecker et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Characterization of cross-primed spacer acquisition in the two Lens CRISPR-Cas systems. The experimental set-up is the same as described in Figure 5. All data are the average of three biological replicates. The distribution of newly targeted protospacers mapped to the priming plasmid reveals cross-priming between the Lens (chromosome) and Lens (pLPL) CRISPR-Cas systems. (A) shows Lens (chromosome) primed, Lens (pLPL) array examined while (B) shows Lens (pLPL) primed, Lens (chromosome) array examined.