Table 1. Description of comparisons for gene expression analyses. The comparisons are pairwise, with condition 1 and 2 indicated.

1: Virgins with and without a germline
Test: Impact of female germline in virgins
Condition 1	Condition 2
Virgin tud/+ (no germline)	Virgin tud/+ (germline)
2: Virgin vs. 1- and 3- day post-mating
Test: Impact of female germline and mating
Condition 1	Condition 2
Virgin tud/+ (germline)	Mated tud/+ (germline) female to Berlin male
Virgin tud/+ (no germline)	Mated tud/+ (no germline) female to Berlin male
3: Virgin vs. 1- and 3- day post-mating
Test: Impact of male germline and mating
Condition 1	Condition 2
Virgin Berlin	Mated Berlin female to tud/+ (germline) male
Virgin Berlin	Mated Berlin female to tud/+ (no germline) male