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. 2020 Jan 3;10(3):985–997. doi: 10.1534/g3.119.401033

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Copyright © 2020 Camino et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Comparison of fluorescence properties of various fluorescent reporters when regulated by an enhancer. Transgenic D. melanogaster were made that possessed the yBE0.6 enhancer driving the expression of fluorescent reporters with an Hsp70 minimal promoter. The reporters included (A-A’’’) EGFP-NLS, (B-B’’’) mCerulean-NLS, (C-C’’’) mCherry-NLS, and (D-D’’’) E2-Crimson-NLS. For all transgenic fluorescent reporters, male pupae were imaged at settings optimized for blue, green, red, and far-red light.