Table 1. Primers used to clone D. melanogaster enhancers for cloning into reporter transgene vectors.

CRE	∼Size	Primer	Sequence
yBE0.6	600	BE2.5 Fwd	TTCCGggcgcgccCTGTGGGTGCAATGATTTAGAATG
		BE3.5 Rvs	TTGCCcctgcaggGTTATTGGCAGGTGATTTTGAGC
t_MSE2	350	tan_MSE-mid-F	TTCCGggcgcgccTGAAATAATAATAAATAATCAGAAT
		tan_MSE-mid-R	TTGCCcctgcaggTGTTTCAACTCAATCCTAGCAGTTGG
dimorphic	690	DE core Fwd	TTCCGggcgcgccTCGCCTccgcggCTCTTTCTCTTTGCCATTTTAAC
element core		DE core Rvs	TTGCCcctgcaggCCCTTGgctagcGTGTGTGAACCAATTTGTTGTGC
LAE	1,400	bab TRE Fwd	TTCCGggcgcgccGTGAGGGGCAAATTATGGAGAG
		bab TRE Rvs	TTGCCcctgcaggGTGCGCCTAACTAGCCAACAATTAG
Expanded Dimorphic	1,580	sub1orthoF1	TTCCGggcgcgccCACATAAAAATCAGCAACAAASTTGC
Element		dimorphic Rvs1	TTGCCcctgcaggCAAAACKGCRCATAAAAMSAAATTACA

Notes:

^1.‘ggcgcgcc’ and ‘cctgcagg’ are sequences recognized respectively by the AscI and SbfI restriction endonucleases. These restriction enzyme sites were used to clone PCR amplified sequences into the pRLGL8 reporter vector. ‘ccgcgg’ and ‘gctagc’ are sequences recognized respectively by the SacII and NheI restriction endonucleases.

^2.The approximate PCR product sizes are reported in base pairs (bp).

^3.‘S’, ‘K’, ‘R’, and ‘M’ are IUPAC notations for degenerate bases. S = G or C, K = G or T, R = A or G, and M = A or C.