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. 2020 Jan 9;10(3):881–890. doi: 10.1534/g3.119.400716

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Copyright © 2020 Susic et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Maximum likelihood phylogenetic tree based on the 8 available genomic assemblies of Bacillus firmus and Bacillus oceanisediminis 2691 with heatmap annotation. Nodes annotated with bootstrap support. Average nucleotide identity (ANI) values (%) are presented in a heatmap, ranging from lower (violet) to higher sequence identity (green-yellow), and clustered according to the phylogenetic tree. ANI analysis showed 100% sequence identity between B. firmus NBRC 15306 and B. firmus NCTC 10335, which is in accordance with information from the NCBI GenBank (data not shown). B. firmus I-1582 exhibited 98.14% sequence identity with strains NBRC 15306 and NCTC 10335 (highlighted in red), and 89.83% sequence identity with Bacillus sp. ZZV12-4809. The heatmap was annotated with a bar chart showing the varying sizes (Mb) of all 8 assemblies (at the top) and their respective GC (%) content (right-hand side).