Figure 4.
TUG-891 increases [Ca2+]i in mouse AM. (a–f) The representative recording of Fluo-3 fluorescent intensity in AM in controls with 0.1% DMSO vehicle, TUG-891 treatment, YM-254890 treatment+TUG-891, U73122 treatment+TUG-891, thapsigargin treatment+TUG-891, and EGTA treatment+TUG-891, respectively. The statistic of the average fluorescent intensity in macrophages in 10 min from TUG-891 or vehicle treatment is shown in (g), and the statistical analysis of inhibition of TUG-891-stimulated increase in [Ca2+]i by the Gq protein inhibitor YM-254890, the PLC inhibitor U-73122, depletion of Ca2+ store by thapsigargin, and removal of extracellular Ca2+ is shown in (h). ∗∗P < 0.01vs. the control, n = 20.