Figure 5.

The correlation between miR-215-5p and ZEB2 in podocyte migration. (a) Targeted binding sites between miR-215-5p and ZEB2-3′-UTR sequences were predicted by TargetScan database and effect of miR-215-5p on transcription activity of ZEB2 determined by luciferase report assay in 293 cells. (b) The expression of ZEB2 was measured by qRT-PCR in 293 cells treated with miR-215-5p mimic. (c) The protein level of ZEB2 was measured by western blotting in MPC5 cells treated with miR-215-5p mimic. (d) Grayscale quantitation of ZEB2 protein expression. ^∗P < 0.05; ^∗∗P < 0.01.