Table 3. Specific primers used for real time quantitative PCR.
| Gene name | Sequence | Ta | Product size (BP) | Gene bank accession no |
|---|---|---|---|---|
| 18S rRNA | Forward:
5-GCGGCTTTGGTGACTCTA-3 Reverse: 5-CTGCCTCCTTGGATGTG-3 |
60 | 194 | NR 002170.3 |
| Acetyl CoA carboxylase | Forward: 5-ATG TTT CGG CAGTCC CTG
AT-3 Reverse: 5-TGT GGA CCA GCTGAC CTT GA-3 |
60 | 133 | EF618729 |
| Fatty acid synthase | Forward: 5-AGC CTA ACT CCTCGC TGC
AAT-3 Reverse: 5-TCC TTG GAA CCGTCT GTG TTC-3 |
58 | 196 | AY183428 |
| Lipoprotein lipase | Forward: 5-AAC TTG TGG CTGCCC
TAT-3 Reverse: 5-GAC CCT CTG GTGAAT GTG-3 |
55 | 367 | X62984 |
| Hormone sensitive lipase | Forward: 5-GCT CCC ATC GTCAAG AAT
C-3 Reverse: 5-TAA AGC GAA TGCGGT CC-3 |
55 | 262 | AJ000482 |
RNA, ribonucleic acid.