Figure 5.

Effects of a PKC inhibitor on the dendritic development of primary cultured Purkinje cells. A, Purkinje cells from WT and DGKγ KO mice were cultured for DIV21. The cells were treated with the PKC activator TPA, the panPKC inhibitor GF109203X (GFX) and the cPKC inhibitor Gö6976 (Gö) for the final 3 d. After fixation, the cells were immunostained with an anti-calbindin antibody. Scale bar: 50 μm. B, A comparison of the number of dendrites and branches and the total length of primary cultured Purkinje cells from WT and DGKγ KO mice (WT, control (con): n = 31, TPA: n = 22, GFX: n = 27, Gö: n = 22; KO, con: n = 31, TPA: n = 26, GFX: n = 27, Gö: n = 24); *p < 0.05, **p < 0.01, followed by Tukey’s multiple comparisons test. Data are expressed as mean ± SEM.