Figure 8.

Rescue of motor coordination in DGKγ floxed (tm1c) mice. A, WT, tm1a, and tm1c alleles. B, Typical results of PCR genotyping. Bands at 975, 907, and 523 bp were expected for the tm1c, WT, and tm1a alleles, respectively. C, Cerebral and cerebellar lysates from WT, tm1a (1a), and tm1c (1c) mice were subjected to Western blotting and probed with anti-DGKγ and anti-GAPDH antibodies. D, Motor coordination of WT, tm1a (1a), and tm1c (1c) mice was assessed by the accelerating rotarod test (WT: n = 14; 1a: n = 15; 1c: n = 15); *p < 0.05, **p < 0.01, followed by Tukey’s multiple comparisons test. E, Motor coordination of WT, tm1a (1a), and tm1c (1c) mice was assessed by the number of hind paw slips in the beam test (WT: n = 5; 1a: n = 5; 1c: n = 3); *p < 0.05, followed by Tukey’s multiple comparisons test. Data are expressed as mean ± SEM.