Figure 3.

METTL3 Accelerates the Proliferation, Invasion, and Migration of OSCC Cells

(A) Plasmids of METTL3 silencing or overexpression were transfected to establish the stable transfection in SCC25 cells and CAL27 cells. qRT-PCR analysis detected the METTL3 mRNA expression. (B) Western blot analysis showed the protein levels of METTL3 in SCC25 and CAL27 cells transfected with METTL3 shRNA or overexpression plasmids. (C) EdU assay was performed to illustrate the proliferation ability of OSCC cells. (D) Wound-healing assay illustrated the migration of OSCC cells. (E) Transwell assay elucidated the migration of OSCC cells in SCC25 and CAL27 cells transfected with METTL3 shRNA or overexpression plasmids. (F) Transwell assay elucidated the invasion of OSCC cells. Three independent experiments were performed. *p < 0.05 versus control; **p < 0.01 versus control.