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. 2020 Feb 4;20:1–12. doi: 10.1016/j.omtn.2020.01.033

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© 2020 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

YTHDF1 Mediates the c-Myc Stability through an m⁶A-Dependent Manner

(A) In the public database (GEPIA), the expression of YTHDF1 was positively correlated with that of c-Myc. (B) Western blot analysis showed the c-Myc protein expression in SCC25 and CAL27 cells transfected with YTHDF1 silencing or YTHDF1 overexpression. (C) RNA immunoprecipitation following qPCR (RIP-qPCR) illustrated the direct interaction of YTHDF1 targeting c-Myc mRNA. (D) After actinomycin D (Act-D; 5 μg/mL), RNA stability assay showed the mRNA half-life (t_1/2) of SCC25 and CAL27 cells transfected with YTHDF1 silencing or YTHDF1 overexpression. (E) RIP-qPCR unveiled the interaction within YTHDF1 and c-Myc mRNA after METTL3 inhibition. Three independent experiments were performed. **p < 0.01 versus control.