FIGURE 1.

A schematic representation of the antibiotic treatment schedule and analysis in murine models of acute and chronic Pseudomonas aeruginosa infection. At day 0, mice were infected with P. aeruginosa planktonic cells to mimic acute infection or with P. aeruginosa embedded in agar beads to achieve long-term chronic infection. In the acute infection model, the treatment schedule used with the antibiotics was a single dose administered 5 min after infection by aerosol, intranasal or subcutaneous routes. In the chronic infection model, the treatment started 5 min (“early” treatment) or 7 days (“late” treatment) after infection, with repeated daily doses for 7 days. Read-outs of the disease progression were body weight changes, colony-forming units (CFUs), total and differential cells, and cytokines/chemokines assayed at the time of death (at 6 h for acute infection or after 7 days of treatment for chronic infection). d: days; BALF: bronchoalveolar lavage fluid.