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In vitro Cd binding assay. (A) Escherichia coli trigger factor (TF) and TF fused to the N terminus of the signal peptide-deleted CAL2 (TF-ΔSPCAL2) purified from E. coli. (B) The molar ratio of Cd against TF-ΔSPCAL2 protein purified from E. coli cells grown with 100 μM CdCl2. Data are mean ± standard deviations of three independent biological replicates. Significant differences were determined by Student’s t-test (**P < 0.01).
