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. 2020 Feb 27;11:217. doi: 10.3389/fpls.2020.00217

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Copyright © 2020 Luo, Xiao, Yao, Wu, Yang, Ismail and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Tissue specificity expression and subcellular localization assay. (A,B) Histochemical assay of CAL2 promoter GUS activity in 14-day-old rice seedlings. (C,D) Subcellular localization of CAL2 in Arabidopsis (C) and rice (D) plants harboring the construct 35S:CAL2-mRFP. 7-day-old seedling root epidermal cells were incubated in 40% sucrose to induce plasmolysis and then imaged by confocal microscopy. The arrows indicate the plasmolyzed cells. Scale bars = 2 mm in (A), 1 cm in (B), and 20 μm in (C,D). Two independent transgenic lines were used in (A–D) and showed similar results.