FIGURE 5.
Ectopic expression of CAL2 increased Cd allocation to shoot in Arabidopsis. Four weeks old 35S:CAL2-mRFP transgenic plants were exposed to 10 μM Cd for 3 days. (A) Heterologous overexpression of CAL2 increased Cd concentration in Arabidopsis shoots. (B) Heterologous overexpression of CAL2 decreased Cd concentration in Arabidopsis roots. Data are mean ± standard deviations of three independent biological replicates. Significant differences from Col-0 were determined by Student’s t-test (*P < 0.05, **P < 0.01).