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. 2020 Feb 27;11:217. doi: 10.3389/fpls.2020.00217

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Copyright © 2020 Luo, Xiao, Yao, Wu, Yang, Ismail and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cd tolerance assays of CAL2 overexpression lines. Transgene-negative control ZH11 and 35S:CAL2-mRFP transgenic rice seedlings were grown for 14 days in hydroponics supplemented with 0 or 5 μM Cd, and then sampled to determine fresh weights (FW) of shoot (A) and roots (B), Cd concentration in leaves (C), xylem sap (D) and roots (E) were determined by ICP-MS. Data are mean ± SDs of three independent biological replicates. Bars with the same letters are not significantly different at P < 0.05 using the LSD method. Significant differences from ZH11 were determined by Student’s t test (^∗P < 0.05).