Table 3.

Reagents details.

Antibodies used for immunocytochemistry/flow-citometry
	Antibody	Dilution	Company Cat # and RRID
Pluripotency Markers	Mouse anti-OCT4	1:100	Santa Cruz Biotechnology Cat# sc-5279, RRID:AB_628,051
Pluripotency Markers	Rabbit anti-SOX2	1:200	Abcam cat# ab97959 AB_2,341,193
Pluripotency Markers	Rabbit anti-NANOG	1:200	Abcam cat# ab80892 RRID: AB_2,150,114
Differentiation Markers	Rabbit anti- SOX17	1:100	Millipore cat#09–038 RRID: AB_1,587,525
Differentiation Markers	Mouse anti-Actin, α-smooth muscle	1:300	Sigma cat# A2547 RRID: AB_476,701
Differentiation Markers	Mouse anti class III beta-Tubulin	1:100	Stemcell Technologies cat# 1409 RRID: AB_215,509
Secondary antibodies	Donkey Anti-Mouse IgG, Alexa Fluor 546	1:500	Thermo Fisher Scientific Cat# A10036, RRID:AB_2,534,012
Secondary antibodies	Donkey Anti-Rabbit IgG, Alexa Fluor 647	1:500	Molecular Probes Cat# A-31,573, RRID:AB_2,536,183
Secondary antibodies	Donkey Anti-Mouse IgG, Alexa Fluor 488	1:500	Molecular Probes Cat# A-21,202, RRID:AB_141,607

Primers
	Target	Forward/Reverse primer (5′−3′)
Targeted mutation analysis/sequencing	DMD c.9851G>A (p.Trp3284X) in exon 68	CCAGCCTAGCTTTGCAACCAT / CCCGTGAAGACACGCACT
Targeted mutation analysis/sequencing	DMD c.10141C>T (p.Arg3381X) in exon 70	CCTGGTTTCAGAGCCCCATT / TGGCAACTGGACATCAGCTT
House-Keeping Gene (qPCR)	GAPDH	TTCACCACCATGGAGAAGGC/ GGCATGGACTGTGGTCATGA
Reprogramming factor (qPCR)	NANOG	CAATGGTGTGACGCAGGGAT/ CCAAGTCACTGGCAGGAGAAT
Reprogramming factor (qPCR)	SOX2	AACCAGCGCATGGACAGTTA/ GACTTGACCACCGAACCCAT
Reprogramming factor (qPCR)	OCT3/4	AGGTTTCTCACCTGTGTGGGTT/ CTTTGTGTTCCCAATTCCTTCC