Figure 2.
MHC I Molecules Show a Similar TAPBPR Binding Hierarchy in a Cellular System
(A) Bar graphs show surface expression levels of each HLA I allotype upon reconstitution in HeLaM-HLA-ABCKO cells.
(B) Histograms depict levels of soluble TAPBPR bound to each HLA I allotype present at the cell surface, after cells were incubated with 1 μM TAPBPR for 30 min at 37°C; HLA I allotypes were grouped into either strong (blue) or weak (red) TAPBPR binders. HLA I-deficient cells were shown in gray.
(C) Bar graph summarizing TAPBPR binding measured as in (B).
Error bars show MFI ± SD from three independent experiments.