Figure 3.

Intracellular Species of HLA I Molecules Confirm TAPBPR-Binding Hierarchy while Revealing Broader Reactivity to TAPBPR

Western blot analysis on (A) recombinant TAPBPR pull-downs from the lysate of each HLA I-reconstituted HeLaM-HLA-ABC^KO cell line and on (B) endogenous TAPBPR and tapasin immunoprecipitates. Membranes were probed with antibodies specific for MHC I heavy chain (using HC10 and HCA2), TAPBPR, tapasin, β₂m, and calnexin, as indicated. These are representative examples of three independent experiments.