Figure 7.

M12 Residue, Found Specifically in HLA-A^∗68:02, Promotes Its Accessibility to TAPBPR

(A) PyMOL figures highlighting amino acid differences between A^∗68:02 and A^∗68:01 (left), A^∗69:01 (center), and A^∗02:01 (right), respectively; conserved residues are colored in blue, different residues between A^∗68:02 and A^∗68:01 in red, and differences between A^∗68:02 and other strong binders in green.

(B) Histograms showing cell surface levels of A^∗68:02^WT, A^∗68:02^M12V, A^∗68:02^P105S, A^∗02:01^WT, A^∗02:01^V12M, and A^∗02:01^S105P.

(C) Histograms show TAPBPR binding to the HLA I variants in (B), after cells were treated with 100 nM TAPBPR for 30 min.

(D) Bar graphs showing TAPBPR binding to the HLA I allotypes tested in (C), upon treatment with either 100 nM (light gray) or 1 μM TAPBPR (dark gray).

Bars show MFI ± SD from three independent experiments. n/s, not significant; ^∗p ≤ 0.05, ^∗∗∗∗p ≤ 0.0001 using unpaired two-tailed t test.