Table 2. Specificity, Digestibility, Recovery, and Stability of Fully Tryptic Peptides with 5–21 Amino Acids in Peptide Selection (n = 3)^a.

		specificity			digestibility			recovery			stability
number	sequence	hit number	alignment score	grades	hydrolysis ratio in standard (%)	hydrolysis ratio in matrix (%)	grades	recovery rate in dimethylation (%)	recovery rate in matrix (%)	grades	reproducibility in dimethylation (%)	reproducibility in matrix (%)	rrades
1	SEIAHR	21	22.3	L	69.01	61.77	L	95.08	104.55	H	8.54	2.92	M
2	DLGEEHFK	2	29.5	M	91.41	94.69	M	92.29	100.52	H	4.01	3.07	H
3	GLVLIAFSQYLQQCPFDEHVK	1	72.3	M	100.52	97.04	H	101.18	523.13	L	30.29	49.72	L
4	LVNELTEFAK	0	34.6	H	100.76	99.19	H	98.70	93.47	H	2.59	4.39	H
5	TCVADESHAGCEK	0	44.8	H	102.99	94.40	M	93.08	90.33	H	2.47	1.33	H
6	SLHTLFGDELCK	1	41.8	M	98.70	94.75	M	97.34	93.23	H	3.49	3.09	H
7	VASLR	99	17.6	L	99.39	98.38	H	98.63	98.69	H	2.00	1.64	H
8	ETYGDMADCCEK	1	45.2	M	68.48	85.58	L	91.75	94.54	H	3.04	3.42	H
9	QEPER	83	20.2	L	ND	ND	ND	149.50	296.62	L	45.50	40.17	L
10	NECFLSHK	0	30.3	H	0.00	0.00	L	88.72	104.15	M	19.99	13.53	L
11	DDSPDLPK	1	28.6	M	39.03	27.10	L	92.16	104.52	H	3.96	7.26	M
12	LKPDPNTLCDEFK	0	46.4	H	20.90	12.43	L	86.66	84.69	L	2.40	2.74	H
13	YLYEIAR	6	27.4	M	101.47	98.48	H	100.39	101.42	H	1.96	2.87	H
14	HPYFYAPELLYYANK	1	55.4	M	83.08	91.88	L	99.75	179.91	L	11.56	6.15	L
15	YNGVFQECCQAEDK	1	51.1	M	18.97	33.39	L	89.16	89.56	M	5.97	5.92	L
16	GACLLPK	1	24.8	M	41.66	75.52	L	93.81	97.21	H	3.05	1.86	H
17	IETMR	91	21.0	L	100.01	95.64	H	98.84	100.24	H	1.91	1.75	H
18	VLASSAR	1	22.7	M	ND	ND	ND	ND	ND	ND	ND	ND	ND
19	CASIQK	4	22.3	M	95.97	88.35	L	93.77	88.72	M	2.48	1.34	H
20	AWSVAR	6	22.7	M	99.55	98.96	H	96.79	99.20	H	2.33	2.14	H
21	AEFVEVTK	0	28.2	H	99.22	101.04	H	95.26	34.18	L	1.90	2.02	H
22	LVTDLTK	5	24.4	M	100.32	97.71	H	95.15	88.55	M	2.22	1.88	H
23	ECCHGDLLECADDR	15	51.1	L	8.12	0.00	L	89.56	119.84	L	7.21	11.25	L
24	ADLAK	99	17.6	L	28.25	92.90	L	88.90	103.35	M	3.48	6.70	M
25	YICDNQDTISSK	0	43.1	H	100.81	91.99	M	95.25	91.57	H	3.12	2.22	H
26	ECCDKPLLEK	3	37.1	M	84.24	101.34	L	84.28	83.83	L	5.42	3.48	M
27	SHCIAEVEK	0	32.5	H	40.99	37.45	L	89.21	124.27	L	5.31	5.69	M
28	DAIPENLPPLTADFAEDK	0	60.4	H	ND	ND	ND	ND	ND	ND	ND	ND	ND
29	NYQEAK	4	23.1	M	100.44	92.81	M	94.80	91.88	H	2.32	2.02	H
30	DAFLGSFLYEYSR	0	45.6	H	98.42	83.72	L	93.29	86.24	M	6.45	15.00	L
31	HPEYAVSVLLR	2	38.4	M	76.69	21.85	L	95.45	125.21	L	6.49	5.80	M
32	EYEATLEECCAK	0	43.5	H	33.75	48.14	L	92.04	95.93	H	3.03	3.54	H
33	DDPHACYSTVFDK	0	46.9	H	0.02	0.00	L	93.83	96.17	H	3.13	4.97	H
34	HLVDEPQNLIK	2	39.2	M	101.09	96.85	H	97.14	93.20	H	2.44	1.90	H
35	QNCDQFEK	0	31.2	H	98.45	95.36	H	90.61	87.33	M	2.88	2.96	H
36	LGEYGFQNALIVR	2	44.8	M	99.32	32.05	L	132.10	167.25	L	85.34	62.16	L
37	VPQVSTPTLVEVSR	5	46.0	M	100.70	31.05	L	103.79	90.50	H	5.88	5.61	M
38	CCTKPESER	0	33.7	H	96.62	89.64	L	91.45	87.38	M	5.85	4.75	M
39	MPCTEDYLSLILNR	1	51.1	M	97.32	95.58	H	92.71	108.15	M	7.43	2.72	M
40	LCVLHEK	12	26.5	L	101.11	94.05	M	89.88	87.27	M	2.86	2.30	H
41	TPVSEK	23	21.4	L	97.09	94.82	M	96.92	94.09	H	5.97	2.95	M
42	CCTESLVNR	7	33.3	M	102.25	95.70	H	92.89	95.08	H	2.97	2.81	H
43	RPCFSALTPDETYVPK	0	55.8	H	101.31	90.20	M	98.38	93.05	H	5.06	2.23	M
44	AFDEK	60	19.3	L	98.29	97.53	H	91.04	89.08	M	3.24	2.09	H
45	LFTFHADICTLPDTEK	0	56.2	H	97.47	96.61	H	85.09	100.20	M	6.74	2.73	M
46	QTALVELLK	4	30.3	M	99.60	97.01	H	97.54	94.51	H	2.42	2.69	H
47	ATEEQLK	8	24.8	M	99.64	97.84	H	93.15	89.56	M	1.92	1.34	H
48	TVMENFVAFVDK	1	43.1	M	95.43	93.45	M	93.06	97.37	H	5.03	3.98	M
49	CCAADDK	2	26.5	M	ND	ND	ND	106.07	154.49	L	60.05	77.46	L
50	EACFAVEGPK	2	35.0	M	22.97	10.29	L	94.66	108.06	M	5.25	7.66	M
51	LVVSTQTALA	0	32.0	H	100.12	102.89	H	94.96	102.04	H	4.03	3.57	H

^a*ND: not detected.