Table 3. Method Validation and Comparison of the Two Peptide Selection Methods (n = 3).

					recovery (%)	RSD (%)
peptides	linearity	LOD (mg/kg)	LOQ (mg/kg)	spiked level (mg/kg)	intraday		interday	BSA content (mg/kg)
LVNELTEFAK	Y = 1.27059*X – 1.05696	0.78	2.59	50	96.43	4.22	8.18	169.3 ± 8.0
				200	104.92	1.78	7.77
	R = 0.9995			500	104.08	2.58	6.74
YLYEIAR	Y = 1.11458*X – 0.401474	0.78	2.59	50	94.77	3.65	9.43	170.5 ± 3.7
				200	104.06	3.18	9.84
	R = 0.9999			500	103.17	4.46	9.83
QTALVELLK	Y = 1.4148*X – 1.08762	1.99	6.63	50	97.25	3.09	9.36	161.2 ± 6.1
				200	96.91	4.73	8.24
	R = 0.9996			500	98.11	4.43	9.36
AEFVEVTK	Y = 1.15335*X – 0.689148	0.86	2.88	50	88.03	4.03	8.44	152.5 ± 6.0
				200	98.22	3.47	7.54
	R = 0.9998			500	102.63	4.73	9.47
LGEYGFQNALIVR	Y = 0.239964*X – 0.320657	15.6	52.0	50	102.21	17.91	46.66	121.4 ± 60.3
				200	101.44	22.40	43.47
	R = 0.9969			500	102.89	16.07	83.21